1. Choose columns based on functions:
Normal phase: solvent soluble solutions
Reverse phase: hydrocarbon soluble solutions
Ion-exchange: separate monosaccharides
Size-exclusion: separate proteins and carbohydrates
2. Diameter x Length vs Pressure:
Shorter and wider columns cut analysis time and require less pressure to move the mobile phase.
Longer and narrower columns provide higher resolution and more sensitive results. They require less samples for analysis but a higher pressure.
...continue reading below the images
Replacement Nut, Metal, For Split/Splitless I More...
Sigma Aldrich Ascentis® Express 90 Å PFAS, (2 More...
HPLC Column, 10 µm, 4.1 ID x 100 mm L, More...
NT Cartridge Holder, For 2, 3, 4 and 4.6 mm I More...
Guard Cartridge Holder, Analytical Column Siz More...
Analytical Guard Cartridge Starter Kit, 10 &m More...
Sigma Aldrich Ascentis® C18 (3 µm) HPLC colum More...
HPLC Column, 9 µm, 7.8 ID x 305 mm L, S More...
GC Column, 0.32 mm ID x 60 m L, Fused Silica More...
HPLC Column, 5 µm, 4.1 ID x 50 mm L, St More...
Capillary Tubing, 5 m L, 0.53 ID x 0.68 mm OD More...
Sigma Aldrich Ascentis® Express 90 Å PFAS, (2 More...
3. Column Material:
Stainless steel is the material of choice for packed columns in high pressure (HPLC) analysis
Capillary columns are much thinner because the stationary phase is chemically bonded to their inner wall coating.
We recommend wall-coated open tubular (WCOT) capillary columns with fused silica lining tubes for gas chromatography (CG). Because of their superior strength and flexibility.
We have 80+ year relationships with many of your preferred manufacturers. Which means you get realistic and honest ETAs on product availability.
If the supply chain doesn't work with your timeline, we can find good alternatives that fit your need. So you don't have to waste time searching.